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Methods

Explore methods developed by and/or used in VHP4Safety.

You can search for a method that you know using the search box below. Alternatively, you can filter the methods with respect to the case studies' flow step that they belong to and/or the regulatory question that they are associated with. Use the "Filters" button to show filter options, then "Apply Filter" to see results, and click "Clear Filter" to reset the filters.

    H2DCFDA-AM assay for detection of oxidative stress production

    Fluorescence-based detection of reactive oxygen species production

    Collagen I Polyclonal Antibody

    immunofluorescent detection of collagen - 1

    Intracellular measurements

    Intracellular measurements are determined in cell lysates using LCMS, normalized by protein expression that has been measured by 2D Quant

    KIM-1 staining

    KIM-1 staining is an immunofluorescent staining of kidney injury molecule 1, an protein that is expressed when kidney injury takes place

    Lactate assay

    Detection of L-lactate in biological samples

    LDH activity assay to determine cell death

    Colorimetric determination of lactate dehydrogenase (LDH) activity