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Methods

Explore methods developed by and/or used in VHP4Safety.

You can search for a method that you know using the search box below. Alternatively, you can filter the methods with respect to the case studies' flow step that they belong to and/or the regulatory question that they are associated with. Use the "Filters" button to show filter options, then "OK" to see results, and click "X" to reset the filters.

    LysoTracker Green DND-26

    Fluorescent - based detection of lysosomes

    P-gp Staining

    Immunofluorescent staining of P-gp protein, the efflux transporter of tacrolimus

    MitoProbe™ JC-1 Assay Kit

    mitochondrial membrane potential assay

    MitoTracker Deep Red FM

    Fluorescent-based assay to label mitochondria

    P-gp Staining

    Immunofluorescent staining of P-gp protein, the efflux transporter of tacrolimus

    RT-qPCR

    Technology used for measuring RNA using PCR

    Resazurine assay

    Fluorometric method to measure cell viability through enzymatic reduction of resazurin to resorufin by viable cells

    RNA-sequencing

    next-generation sequencing technique used to quantify and identify RNA molecules in a biological sample