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TempO-Seq transcriptomics of iPSC-derived proximal tubular-like and endothelial-like cell mono- and co-cultures

TempO-Seq transcriptomics was used to characterise iPSC-derived proximal tubular-like cell (PTL) and endothelial-like cell (ELC) mono- and co-cultures in either static or organ-on-a-chip conditions, to assess whether these culture conditions could enhance the maturity and function of PTL and ELC. Both cell types were differentiated from the human induced pluripotent stem cell line SBAD3-01 (STBCi322-A). Untreated cultures were profiled across nine conditions — PTL and ELC mono-cultures and PTL/ELC co-cultures (on the same filter, or on filter and well), under static or microfluidic organ-on-a-chip conditions, with three biological replicates each — using the BioSpyder TempO-Seq Human Whole Transcriptome assay (~22,500 target genes), with gene expression quantified as mRNA read counts.

Details
Type
study
Internal ID
S-VHPS17
Release date
2026-06-01
Version
—
License
CC-BY
URL
https://www.ebi.ac.uk/biostudies/studies/S-VHPS17
DOI
10.6019/S-VHPS17
Publications

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Files

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Authors
Funding
  • Dutch Research Council (NWO)
    1292.19.272
Proximal tubular-like cells (PTL-SBAD3-01)
RRID: CVCL_ZX55 · Supplier: StemBANCC
Homo sapiens · Kidney · Proximal tubule · Epithelial cell of proximal tubule

Proximal tubular-like cells differentiated from the human induced pluripotent stem cell line SBAD3-01 (Cellosaurus STBCi322-A, CVCL_ZX55; original iPSC source StemBANCC), used as the epithelial compartment of an iPSC-based kidney proximal tubule model.

Endothelial-like cells (ELC-SBAD3-01)
RRID: CVCL_ZX55 · Supplier: StemBANCC
Homo sapiens · Kidney · Vascular endothelium · Endothelial cell

Endothelial-like cells differentiated from the human induced pluripotent stem cell line SBAD3-01 (Cellosaurus STBCi322-A, CVCL_ZX55; original iPSC source StemBANCC), used as the vascular/endothelial compartment of the iPSC-based kidney model.

No chemical information available.

No exposure information available.

Bioassays

Coming soon: normalization, model fitting, statistics, QC, etc.

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