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Exposure matters: the impact of in vitro exposure scenarios

This study examines how different in vitro exposure scenarios influence the toxicity of pesticides in differentiated SH-SY5Y dopaminergic-like cells. Cells were exposed under varying scenarios — single 24 h exposures with different delays before measurement (0, 18, 72 h), a repeated exposure (4 × 6 h) and a prolonged 96 h exposure — and two endpoints were measured in parallel: mitochondrial metabolic activity (alamarBlue) and cytotoxicity (lactate dehydrogenase release).

Details
Type
study
Internal ID
S-VHPS16
Release date
2026-05-25
Version
—
License
CC-BY
URL
https://www.ebi.ac.uk/biostudies/studies/S-VHPS16
DOI
10.6019/S-VHPS16
Publications

No linked publications available.

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Authors
Funding
  • Dutch Research Agenda (NWA)
    1292.19.272
SH-SY5Y
RRID: CVCL_0019 · Supplier: VUmc
Homo sapiens · Brain · Substantia nigra pars compacta · Neuroblastoma-derived cell

Human SH-SY5Y neuroblastoma cells differentiated to a dopaminergic-like phenotype, used as an in vitro model of substantia nigra dopaminergic neurons.

Structure for CID 6950
Dinoseb
CAS: 88-85-7
PubChem: CID 6950
CompoundCloud: Q4723
Structure for CID 3224
Endosulfan
CAS: 115-29-7
PubChem: CID 3224
CompoundCloud: Q4727
Structure for CID 3034368
Mancozeb
CAS: 8018-01-7
PubChem: CID 3034368
CompoundCloud: Q4734
Structure for CID 6758
Rotenone
CAS: 83-79-4
PubChem: CID 6758
CompoundCloud: Q38
Exposure type: acute · Exposure duration: 24 hours · Exposure-measurement interval: 0 hours
Exposure concentration: 0.1, 0.3, 1, 3, 10, 30, 100 (plus 0 µM vehicle control) µM · Bioassays: Alamar blue, Lactate dehydrogenase (LDH)
Exposure type: recovery · Exposure duration: 24 hours · Exposure-measurement interval: 18 hours
Exposure concentration: 0.1, 0.3, 1, 3, 10, 30, 100 (plus 0 µM vehicle control) µM · Bioassays: Alamar blue, Lactate dehydrogenase (LDH)
Exposure type: delayed · Exposure duration: 24 hours · Exposure-measurement interval: 72 hours
Exposure concentration: 0.1, 0.3, 1, 3, 10, 30, 100 (plus 0 µM vehicle control) µM · Bioassays: Alamar blue, Lactate dehydrogenase (LDH)
Exposure type: repeated · Number of repeats: 4 · Exposure duration: 6 hours · Recovery period: 18 hours · Exposure-measurement interval: 0 hours
Exposure concentration: 0.1, 0.3, 1, 3, 10, 30, 100 (plus 0 µM vehicle control) µM · Bioassays: Alamar blue, Lactate dehydrogenase (LDH)
Exposure type: chronic · Exposure duration: 96 hours · Exposure-measurement interval: 0 hours
Exposure concentration: 0.1, 0.3, 1, 3, 10, 30, 100 (plus 0 µM vehicle control) µM · Bioassays: Alamar blue, Lactate dehydrogenase (LDH)
Bioassays
  • Alamar blue ENM
    Increase, Mitochondrial dysfunction
    Mitochondrial Injury
    Measured: Fluorescence intensity · Readout: Mitochondrial metabolic activity (%) · Replicates: 3 · Technical replicates: 4-6 (4 for 0 µM, 6 for other concentrations) · Positive control: Rotenone (100, 24)
    Assay supplier: Invitrogen
    Assay catalogue number: R12204
    Reagent concentration: 25 µM
    Assay incubation time: 1 hours
  • Lactate dehydrogenase (LDH) BAO
    Increase, Cell death
    Measured: Colorimetric (absorbance) · Readout: Lactate dehydrogenase activity (% of LDH max) · Replicates: 3 · Technical replicates: 4-6 (4 for 0 µM, 6 for other concentrations) · Positive control: Staurosporine (1, 24)
    Assay supplier: Roche
    Assay catalogue number: 11644793001
    Assay incubation time: 30 minutes
AOP Linkage

Coming soon: normalization, model fitting, statistics, QC, etc.

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Parkinson Question 1: Hazard Question 2: Risk Toxicodynamics Adverse Outcome Chemical Characteristics and Hazard Identification